Efficacy of a locally produced microbial phytase from Enterobacter sakazakii ASUIA273 on body weight and hemato-biochemical constituents in broiler chickens

An experiment was carried out in broiler chicks fed different doses of locally produced microbial phytase supplementation to observe their growth performance as health status, and to investigate the changes of hematological and biochemical values. A total of 144 chicks (Cobb) at one-day old were allocated to 4 treatment (T) groups with 12 cages comprising 3 replicates, each cage containing to 12 birds. Experimental formulating diets arranged with 4 levels of 0, 500, 1000 and 1500 phytase enzyme unit (FTU/kg-I) as considered as Th T2, T3, T4 respectively. They were maintained formulating diet on these dietary treatments from 1 to 42 d of age with feed and water made available for ad libitum consumption. At 1 week interval 2 birds from each treatment were weight through out experimental period for assessing the growth performance. For determining the changes of hematological (RBC, Hb, PCV, MCV, MCHC, WBC, Heterophil, Eosinophil, Basophil, Lymphocyte, Monocyte, Thrombocyte and Icterus Index) and biochemical (Albumin, Total Protein, ALT, ALP, AST, GGT, LDH, Cholesterol, Triglyceride, Glucose, Ca, P, Na, K, Cl, Urea, Creatine and Uric acid) values at the age of 6 weeks randomly selected 2 birds were slaughtered and blood were collected. Data were subjected to analysis of variance (ANOVA) using the least significant difference (LSD) by PC-SAS software (SAS Institute, 2009). Data showed that body weight was not affected at periods of 1si and 2nd weeks of age among different treatment groups. But, at ages from 3rd to 6th weeks, weight gains at four treatment gIZ.Qupwsere increased almost sequentially and consistantly, and had been showed more different and significant (p:s 0.05) increased at 4th and 5th weeks of age from the control. No significant and constant treatments effects were observed on blood and biochemical parameters except eosinophil: Accordingly, it can be recommended to use an uncentrifuged microbial phytase in broiler diet during the period from 4th - 5th weeks of age, to achieve increased weight gain without changing hemato-biochemical parameters

Effects of locally produced bacterial phytase on humoral immunity, live body weight and blood characteristics in broilers vaccinated against Newcastle disease

Immune responses in association with body weight performance and hemato-biochemical constituents might influence the Newcastle disease (ND) vaccination by dietary phytase supplementation (Enterobacter sakazakii ASUA273). The objective of the study was to determine the effects of bacterial phytase supplementation on humoral immunity in association with live body weight and blood characteristics of broilers. Male-day-old Cobb broilers (n=180) were assigned into four phytase treatments (0, 500, 1000 and 1500 FTU/kg-1 of diet) with 12 cages comprised of 3 replicates and each cage contained 15 birds. Birds were maintained on formulated basal diet based with available phosphorus (0.19%) that lasted up to six weeks in feed. Birds were vaccinated with a live ND vaccine at day-old and 21 day-old. Two birds were randomly selected from each treatment weekly. Specific antibody to ND, non-specific immunoglobins (IgM, IgG, and IgA) and live body weight were measured weekly. At the end of experiment, the complete haemato-biochemical constituents were determined. Data of humoral immunity with live body weight and haemato- biochemical values were analysed based on factorial arrangement (treatments×weeks) of completely randomized design (CRD), respectively. Response of the humoral immunity shown that although serum-antibody of ND, IgM, and IgG levels were not improved, the mucosal IgA contents were increased with the increasing phytase doses. The live body weights of broilers were significantly increased (P<0.05) to the graded levels of phytase supplementation throughout the experimental period. Cumulative effects of mucosal IgA contents and live body weights of broilers showed significant (P<0.05) interaction between the effects of phytase levels and effects of weeks. Overall, phytase dose at 1500 FTU/kg-1 of diet over the age of 6 weeks-old showed the best performance. Conversely, no significant, sequential, and consistent treatment effects were observed on hemato-biochemical constituents in broiler chickens. It is therefore, concluded that the efficacy of this local phytase was flourished in respective to mucosal IgA contents and live body weights of broilers

Comparison of Near-infrared Spectroscopy with other options for total carotenoids content phenotyping in fresh cassava roots

This study compared the relationship of different phenotyping methods including iCheckTM CAROTENE (iCheck), Chromameter, colour chart and visible/near-infrared spectroscopy (Vis/NIRS) used in quantifying total carotenoids content (TCC) in fresh cassava roots. Using a total of 194 cassava clones harvested from the International Institute of Tropical Agriculture (IITA), Ibadan, we compared the repeatability precision, accuracy of measurement and correlations of these phenotyping methods. From the results, Vis/NIRS-analyzed TCC had high and positive correlations with Chromameter and Color chart (r = 0.91 and 0.71, respectively). On the other hand, the result revealed somewhat moderate correlation (r = 0.67) between Vis/NIRS and iCheck measurements. Vis/NIRS, iCheck and chromameter methods gave high and nearly equal heritability estimates (0.95, 0.98 and 0.98, respectively) illustrating high repeatability precision of these methods; an indication that they can be used for germplasm selection in the early stages of breeding. Conversely, with Bland-Altman plot at 95% confidence level, the accuracy of iCheck was not comparable with that of Vis/ NIRS. The information derived from this analysis directly contributes towards the genetic improvement of root quality traits in cassava and facilitates the sharing of data across cassava breeding consortium

Optimization of Cobalt Nanoparticles for Biogas Enhancement from Green Algae Using Response Surface Methodology

Organic matter may be converted to energy through various methods, but the most preferable one is the Anaerobic Digestion (AD), specifically for biogas production. In sustainable bioenergy production, it can undoubtedly be called one of the most widely used methods from the various feedstock. Over the past years, algae waste has become an increasingly acute environmental problem but luckily it can be used as feedstock to produce bioenergy. In order to improve the energy productivity of green algae, this study is focused on the introduction of cobalt (Co) nanoparticles (NPs) in the AD process. The concentration of Co NPs was optimized using response surface methodology (RSM). Mesophilic temperature range (25–45 °C), initial pH (5–9) and Co NPs dosage (0.5–2 mg/L) were selected as the independent variables for RSM. The results indicated that at optimized values (Co NPs concentration = 1 mg/L, initial pH = 7, and digestion temperature = 35 °C) produced the highest biogas yield of 298 ml. An experiment was carried out at optimized conditions to explore the effect on biogas production. The results showed that Co NPs had a positive influence on biogas yield. The low concentrations achieved higher biogas production as compared to higher ones. A maximum biogas yield of 678 mL is achieved by Co NPs (1 mg/L). AD performance was further evaluated by the modified Gompertz model. Different kinetic parameters were calculated. The values of the performance indicators confirmed that the mathematical model fitted well with experimental data

Statistical modeling and performance optimization of a two-chamber microbial fuel cell by response surface methodology

Microbial fuel cell, as a promising technology for simultaneous power production and waste treatment, has received a great deal of attention in recent years; however, generation of a relatively low power density is the main limitation towards its commercial application. This study contributes toward the optimization, in terms of maximization, of the power density of a microbial fuel cell by employing response surface methodology, coupled with central composite design. For this optimization study, the interactive effect of three independent parameters, namely (i) acetate concentration in the influent of anodic chamber; (ii) fuel feed flow rate in anodic chamber; and (iii) oxygen concentration in the influent of cathodic chamber, have been analyzed for a two-chamber microbial fuel cell, and the optimum conditions have been identified. The optimum value of power density was observed at an acetate concentration, a fuel feed flow rate, and an oxygen concentration value of 2.60 mol m-3, 0.0 m3, and 1.00 mol m-3, respectively. The results show the achievement of a power density of 3.425 W m-2, which is significant considering the available literature. Additionally, a statistical model has also been developed that correlates the three independent factors to the power density. For this model, R2, adjusted R2, and predicted R2 were 0.839, 0.807, and 0.703, respectively. The fact that there is only a 3.8% error in the actual and adjusted R2 demonstrates that the proposed model is statistically significant

Evaluation of humoral immune response, body weight and blood constituents of broilers supplemented with phytase on infectious bursal disease vaccination

Phytase inclusion in phosphorus (P) deficient diet increases the bioavailability of nutrients and plays, indirectly, a role in biological function of many metabolic processes. The possibility of using phytase in diet might influence immune, growth and blood performances of animals. The objective of the study was to assess the effect of local bacterial phytase on humoral immunity in association with weight and blood characteristics of infectious bursal disease (IBD) vaccinated broilers. Male-day-old Cobb-broilers were assigned into four groups based on phytase treatments (0, 500, 1,000 and 1,500 fitase units per kg of diet) with 12 cages comprising three replicates per treatment, each treatment containing 15 birds. They were vaccinated with an IBD vaccine (IBD UPM93) and were fed formulating P (0.19%) deficient diet from 1 to 42 day of age. Results indicated that although serum IBD antibody, IgM, and IgG were not increased, mucosal IgA contents were increased with increasing phytase doses. Data on bird's growth performance revealed that cumulative live weights were increased significantly (p < 0.05) to graded levels of phytase and the highest enzyme level commenced best performance. Results suggest that the local phytase supplementation in low phosphorus diet will improve mucosal IgA contents and body weights of birds

Design and analysis of rectangular microstrip patch antenna at 2.4 and 5 GHz

The future advancements in wireless communication and the network technology is facing new challenges under different circumstances. These challenges are emerging out as revolutionary invention roadmaps. This study strives to contribute in wireless communication and networking systems. A rectangular microstrip patch antenna is proposed. Initially, some common mediums of communication such as WIFI/WLAN and Bluetooth were targeted. The successful execution leads us to target the commons bandwidth of wireless communication i.e. 2.4 and 5 GHz. The dielectric substrate material used for this purpose was FR4 with 1.6 × 38 × 39.8 mm dimensions (thickness × width × height). The design and simulation on HFSS V15.0 revealed the peak at 2.4 and 5 GHz. These results were also verified through 3D polar plot. After successful simulation, the next step was fabrication. DipTrace circuit design tool was used to generate Gerber file and was printed it on glossy paper followed by printing of antenna on substrate. To remove copper surrounding, FeCl3 and butyl solutions were used followed by soldering of SMA, for successful transmission. The simulation results were empirically verified by using VNA (Vector Network Analyzer). The proposed antenna shows significant negative environmental effect on its transmission. The return loss is quantified as -13dB and -27dB at 2.4 and 5 GHz, respectively. In addition, it promises swift and economic transmission of data with minimum noise attenuation requirement

Design and analysis of rectangular microstrip patch antenna at 2.4 and 5 GHz

The future advancements in wireless communication and the network technology is facing new challenges under different circumstances. These challenges are emerging out as revolutionary invention roadmaps. This study strives to contribute in wireless communication and networking systems. A rectangular microstrip patch antenna is proposed. Initially, some common mediums of communication such as WIFI/WLAN and Bluetooth were targeted. The successful execution leads us to target the commons bandwidth of wireless communication i.e. 2.4 and 5 GHz. The dielectric substrate material used for this purpose was FR4 with 1.6 × 38 × 39.8 mm dimensions (thickness × width × height). The design and simulation on HFSS V15.0 revealed the peak at 2.4 and 5 GHz. These results were also verified through 3D polar plot. After successful simulation, the next step was fabrication. DipTrace circuit design tool was used to generate Gerber file and was printed it on glossy paper followed by printing of antenna on substrate. To remove copper surrounding, FeCl3 and butyl solutions were used followed by soldering of SMA, for successful transmission. The simulation results were empirically verified by using VNA (Vector Network Analyzer). The proposed antenna shows significant negative environmental effect on its transmission. The return loss is quantified as -13dB and -27dB at 2.4 and 5 GHz, respectively. In addition, it promises swift and economic transmission of data with minimum noise attenuation requirement

Negative bias temperature instability characterization and lifetime evaluations of submicron pMOSFET

A major effect of different measurement delay in seconds is revealed through quasi DC Stress Measure Stress experiments. We found that different delay of measurements in seconds contributed to different stress time needed to achieve target 10% degradation of Vth. The longer delay, the more time needed for the device to achieve 10% degradation of Vth. The effect on NBTI degradation is shown to be reliant on stress conditions (stress voltage, temperature) and device architecture (gate dimensions, gate oxide thickness). The NBTI lifetime was predicted by extrapolating lifetime to the nominal operating voltage from Time-to-Fail versus stress bias and oxide electric field plots. Both plots show that the lifetime of degradation parameter of Vth is lower compared to the lifetime of degradation parameter of Idsat